Corning® Matrigel® Matrix

£287.43£4,759.59 (Excluding VAT at 20%)

Item Code: DL101-PT Category: Tag: Brand:

Description

Corning extracellular matrices (ECMs) enable researchers to mimic in vivo environments for 2D and 3D cell culture applications.

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumour rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Unique Features

  • Proven and trusted since 1985; most extensively referenced basement membrane matrix
  • Wide selection of basement membranes; used in diverse applications, from cancer and stem cell research to neurobiology
  • Manufactured under stringent quality control practices that exceed industry standards, ensuring protein consistency and superior product performance
  • Rigorous validation for functionality
  • Certified LDEV-free

Applications

Corning Matrigel matrix is effective for the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid and other cell types. These include neurons, Sertoli cells, chick lens, vascular endothelial cells, and hepatocytes.

  • Influencing gene expression in adult rat hepatocytes
  • 3D culture in mouse and human mammary epithelial cells
  • In vivo peripheral nerve regeneration
  • Metabolism and toxicology studies
  • The development of several types of tumour cell invasion assays
  • The in vitro and in vivo study of angiogenesis
  • In vivo propagation of human tumours in immunosuppressed mice
  • The maintenance of human embryonic stem cells and induced pluripotent stem (hiPS) cells
  • Neuronal differentiation of hiPS cells

 

Matrigel Matrix Quality Control Specifications

  • Mouse colonies routinely screened for pathogens via mouse antibody production (MAP) testing
  • LDEV-free EHS tumour
  • Extensive PCR testing performed on a number of pathogens, including LDEV, ensuring strict control of raw materials used during manufacturing
  • Tested and found negative for the presence of bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Gel stability tested for a period of 14 days at 37°C
  • Biological activity determined for each lot using a neurite outgrowth assay; chick dorsal root ganglia, plated on a 1.0 mm layer of Corning Matrigel matrix, required to generate positive neurite outgrowth response after 48 hours without the addition of nerve growth factor

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