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Appleton appGLOW Safe Stain can be used as a direct replacement for hazardous Ethidium Bromide (EtBr) in agarose and polyacrylamide gel electrophoresis. It also has a better sensitivity then EtBr and can detect as little as 0.1ng of DNA.

The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. appGLOW exhibits excitation peaks at 290nm and 490nm, allowing for it to be used with UV and blue light.