Scientists everywhere are already using this exciting evolving technology to generate innovative 3D models never before possible.
- Cells grown in 3D more closely mimic in vivo behaviour in tissues and organs than cells grown in 2D culture models
- 3D environments create more biologically relevant models for research and drug discovery
- Higher success rates for drug compound testing can reduce development costs
|Growth Substrate||Rigid, inert||Mimics natural tissue environment|
|Cell Shape Growth||Loss of cell polarity and altered shape||Similar morphology and polarity|
|Architecture||Less physiological: cells partially interact||Physiological: promotes close interaction between cells, extracellular matrices (ECMs), and growth factors|
|Growth Factor Diffusion||Rapid||Slow: biochemical gradients regulate cell-cell communication and signalling|
|Gene Expression||Different patterns||Maintenance of in vivo expression patterns|
Appleton Woods are able to supply all the tools from Corning you need for the critical steps of 3D cell culture, please click here for an overview of the products we can provide.
One of the most crucial components of 3D cell culture are naturally-derived hydrogels, the most widely used of which is Corning Matrigel matrix. It is rich in laminin, collagen IV, entactin, heparin sulfate proteoglycans, and a number of growth factors, making it ideal to use in applications including cancer and stem cell research. Matrigel matrix is a bioactive matrix which exhibits the mechanical and chemical properties of the in vivo ECM (extracellular matrices).