Blog

3D Cell Culture is here!

Scientists everywhere are already using this exciting evolving technology to generate innovative 3D models never before possible.

Why 3D?

  • Cells grown in 3D more closely mimic in vivo behaviour in tissues and organs than cells grown in 2D culture models
  • 3D environments create more biologically relevant models for research and drug discovery
  • Higher success rates for drug compound testing can reduce development costs
Attribute2D3D
Growth SubstrateRigid, inertMimics natural tissue environment
Cell Shape GrowthLoss of cell polarity and altered shapeSimilar morphology and polarity
GrowthAlteredIn vivo-like
ArchitectureLess physiological:  cells partially interactPhysiological: promotes close interaction between cells, extracellular matrices (ECMs), and growth factors
Growth Factor DiffusionRapidSlow: biochemical gradients regulate cell-cell communication and signalling
Gene ExpressionDifferent patternsMaintenance of in vivo expression patterns

 

Appleton Woods are able to supply all the tools from Corning you need for the critical steps of 3D cell culture, please click here for an overview of the products we can provide.

One of the most crucial components of 3D cell culture are naturally-derived hydrogels, the most widely used of which is Corning Matrigel matrix. It is rich in laminin, collagen IV, entactin, heparin sulfate proteoglycans, and a number of growth factors, making it ideal to use in applications including cancer and stem cell research. Matrigel matrix is a bioactive matrix which exhibits the mechanical and chemical properties of the in vivo ECM (extracellular matrices).

Please contact us at info@appletonwoods.co.uk or on 0121 458 7740 for more information on 3D cell culture products from Corning.

 

March 2019

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