3D Cell Culture is here!

Scientists everywhere are already using this exciting evolving technology to generate innovative 3D models never before possible.

Why 3D?

Cells grown in 3D more closely mimic in vivo behaviour in tissues and organs than cells grown in 2D culture models
3D environments create more biologically relevant models for research and drug discovery
Higher success rates for drug compound testing can reduce development costs

*Attribute*	2D	3D
Growth Substrate	Rigid, inert	Mimics natural tissue environment
Cell Shape Growth	Loss of cell polarity and altered shape	Similar morphology and polarity
Growth	Altered	In vivo-like
Architecture	Less physiological: cells partially interact	Physiological: promotes close interaction between cells, extracellular matrices (ECMs), and growth factors
Growth Factor Diffusion	Rapid	Slow: biochemical gradients regulate cell-cell communication and signalling
Gene Expression	Different patterns	Maintenance of in vivo expression patterns

Appleton Woods are able to supply all the tools from Corning you need for the critical steps of 3D cell culture, please click here for an overview of the products we can provide.

One of the most crucial components of 3D cell culture are naturally-derived hydrogels, the most widely used of which is Corning Matrigel matrix. It is rich in laminin, collagen IV, entactin, heparin sulfate proteoglycans, and a number of growth factors, making it ideal to use in applications including cancer and stem cell research. Matrigel matrix is a bioactive matrix which exhibits the mechanical and chemical properties of the in vivo ECM (extracellular matrices).

Please contact us at info@appletonwoods.co.uk or on 0121 458 7740 for more information on 3D cell culture products from Corning.

March 2019

I am pleased to support Appleton Woods partnership with ZEISS. I recently purchased from Appleton Woods a ZEISS Axiolab 5 compound microscope with a range of bright field and phase contrast objectives and a camera attachment. The service has been excellent. This included a prompt home demonstration by a very knowledgeable representative who also provided some very practical advice with regards to ZEISS objectives.

Dr John S. NoyesScientific Associate

Our account manager Harriet has always been extremely helpful and proactive, helping supply critical items where others have failed. The service is extremely friendly and I always feel the matter is being dealt with behind the scenes, so no need to chase. Always available and responsive to any enquiries we have. Reliable delivery dates in an extremely difficult time for logistics, due to COVID-19, Brexit, and many other factors. Would always be happy to recommend Appleton Woods to other companies in the industry for lab consumables and equipment.

AdamProcurement Officer

Since the start of the Covid-19 pandemic, I have found Appleton Woods to be extremely responsive and reactive to the needs of the University, which is greatly appreciated. Appleton Woods were especially supportive in providing essential supplies for the University PPE Store at a time when it was needed the most.

E. CorkishPurchasing ManagerUniversity of Oxford

I have been ordering from Appleton Woods for a number of years now after our lab started using their molecular reagents. Pricing of most items is very competitive, and delivery has usually been exceptionally quick, even for quite large orders. The local sales reps are also very helpful and responsive, and frequently pay us visits to check on our needs. I will certainly be continuing to order from Appleton Woods.

Dr D. ClarkSenior Research Officer University of Essex

I have been dealing with the company Appleton Woods for several years now and have been extremely satisfied with their level of service. The local representative regularly makes contact with various members of the department with information on new products and special offers that he considers would be of interest and I also receive email updates for circulation. Quotations are sent promptly and orders dealt with efficiently with personal follow ups to ensure we are satisfied with the products. I look forward to a continued association with the company.

T. ClarkeChief TechnicianOxford

Appleton Woods is always the first company I consult when ordering laboratory equipment or consumables. They stock an extensive range of products to suit all needs at prices for every budget. Having liaised with both the customer services team and sales representatives I can say they are always professional, knowledgeable and happy to help. Delivery of items is always very prompt and their regular e-newsletter provides updates of the latest offers and product information. I would definitely recommend this company

Dr R. DavidResearcherLondon

I have been happily working with Appleton Woods for over 7 years in the capacity of laboratory manager encompassing the role of procurement of laboratory consumables and equipment. The product range has always met my needs; there are usually different brands/manufacturers available which either cater for specific needs or small equipment to fit with the available funds - pertinently they offer a much needed budget priced range of items. I have always found them competitive on pricing, which along with their attention to detail, good communication and prompt response time makes them a personal preferred supplier

Alex MartinLab ManagerLondon

The fact that I've been dealing with Appleton Woods for many years says it all - I've never felt the need to take my business elsewhere. The company sells quality products at competitive prices and the service is very reliable. The staff are well informed, helpful and friendly. An excellent organisation

S. ButlerTechnicianOxford

I have found that Appleton Woods are able to provide a wide range of laboratory consumables and equipment at competitive prices. The staff are always very friendly, helpful, and quick to respond to requests. Their reliability means that they are often a first port of call when we need to source new items

K. LangfordResearch TechnicianManchester

Appleton Woods provides an extensive range of laboratory consumables and equipment - both large and small - and on request they make every effort to source spares and replacement parts. The customer service is excellent and the local representative extremely helpful, professional and quick to respond to queries and requests. In addition, Appleton Woods listens to and acts upon customer feedback. This company is on my list of top suppliers of good quality, competitively priced laboratory goods.

Dr S. ShawcrossUniversity of Manchester

Appleton Woods is one of my favourite companies to deal with. The sales staff are always friendly and efficient, providing us with competitive prices on a quote tailored to our needs, as well as regular communication about all current offers, which we have often taken advantage of. Our area sales manager is extremely helpful, sourcing many consumable items for us in the past and is a pleasure to deal with. I would definitely recommend using this company.

J. WoodSenior Technician Institute of Integrative Biology, University of Liverpool

Overall, very pleasant to deal with. Very efficient and competitively priced. I would always recommend this company to anyone.

Dr K. ChamInstitute of Infection and Global Health, University of Liverpool

Appleton Woods have supplied my lab with a wide range of quality consumables and equipment at competitive prices for many years now and the fact that we continue to use them speaks volumes

J. RobinsonSenior TechnicianInstitute of Integrative Biology, University of Liverpool

I've been using Appleton Woods for purchasing laboratory consumables and equipment for a number of years now. The reasons for this are quite simple; an excellent local sales representative who has an in depth knowledge of products, who is always available by telephone or e-mail when further information is required for a particular product and when quotations are required. In addition, prices are competitive and delivery is prompt. Overall, an excellent service. I would definitely recommend this company!

Dr D. HowarthTechnician

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