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Description

Corning® Collagen I, rat tail, 1g (10 x 100mg), is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function.

Source

Rat Tail Tendon

Quality

• Purity = 90% by SDS-PAGE
• Tested for ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells
• Tested for formation of a firm gel up to a dilution of 1:10; further dilution will decrease the rigidity of the gel
• Found negative for bacteria, fungi, and mycoplasma

Formulation

Liquid in 0.02 N acetic acid, concentration range 3-4 mg/ml

Preparation and Storage

Stable for at least three months at 2° to 8°C. Do not freeze.

Handling

Effective as a gel or thin coating at a recommended concentration of 5-10 µg/cm² of growth surface depending on cell type