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Description

Corning® Ultrapure Laminin, mouse, 1mg, is entactic free and is used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.

Quality

• Purity > 95% by SDS-PAGE
• Tested for ability to initiate neurite outgrowth of NG-108 human neuroblastoma cells
• Found negative for bacteria, fungi, and mycoplasma

Source

Engelbreth-Holm-Swarm (EHS) mouse tumor

Molecular Weight

900 kD (unreduced)

Preparation and Storage

Stable for at least three months at -70°C

Handling

Recommended concentration – 1-10 µg/cm² of growth surface depending on cell type

Formulation

Frozen in 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.4