appSCRIPT cDNA synthesis kit, 25 reactions

£95.00 (Excluding VAT at 20%)

Pack Size: 1

Available to Order

Item Code: ARP601 Category: Brand:

Description

  • For reproducible and unbiased cDNA synthesis from 5′ and 3′ ends of mRNAs
  • Contains an ultra-stable reverse transcriptase which can synthesize cDNAs up to 9kb
  • High cDNA yields from as little as 5pg RNA – easily work with low copy templates
  • Broad starting template range – use between 5pg – 5g
  • The kit contains a buffer which has pre-optimised levels of oligo dT and random hexamers which results in reduced transcript bias for downstream qPCR
  • Short protocol (30mins only)

 

Convenient two tube format:

 

  • Tube 1: reverse transcriptase with RNase inhibitor to prevent degradation of RNA by contaminating RNases
  • Tube 2: buffer concentrate containing oligo dT, random hexamers,MgCl2, dNTPs, enhancers and stabilisers

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