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Description

• For reproducible and unbiased cDNA synthesis from 5′ and 3′ ends of mRNAs
• Contains an ultra-stable reverse transcriptase which can synthesize cDNAs up to 9kb
• High cDNA yields from as little as 5pg RNA – easily work with low copy templates
• Broad starting template range – use between 5pg – 5µg
• The kit contains a buffer which has pre-optimised levels of oligo dT and random hexamers which results in reduced transcript bias for downstream qPCR
• Short protocol (30mins only)

Convenient two tube format:

• Tube 1: reverse transcriptase with RNase inhibitor to prevent degradation of RNA by contaminating RNases
• Tube 2: buffer concentrate containing oligo dT, random hexamers,MgCl₂, dNTPs, enhancers and stabilisers