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Description

• Efficient cDNA synthesis and qPCR in a single tube
• Suitable for absolute quantification, gene expression analysis and detection of extremely low copy number targets
• Contains a highly thermostable reverse transcriptase active between 45°C to 55°C
• Reverse transcriptase contains a highly advanced RNase inhibitor to prevent RNA degradation
• qPCR mix contains a hot start polymerase to prevent primer dimers and non-specific products
• Non-PCR inhibiting intercalating dye
• Rapid extension rate for early C_T values
• Advanced sensitivity for increased limits of detection
• Suitable for template amounts of 1pg – 10ng total RNA or >0.01pg mRNA per reaction
• Compatible on the majority of real-time PCR platforms under standard and fast cycling conditions (see selection guide)