appGENE Gel & PCR Clean-up kit, Appleton

Pack Size: 1

Item Code: ARN020-PT Category: Brand:

Description

  • Rapid and efficient purification of plasmid or genomic DNA from enzymatic reactions or from agarose gels
  • Suitable for downstream PCR, qPCR, DNA sequencing, restriction digests, ligation, in vitro transcription, gene expression analysis, and microinjection
  • Remove contaminants like excess primers, unincorporated nucleotides, mineral oil, nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, agarose, and ethidium bromide
  • Purify 100bp to 10kb DNA fragments at 99% recovery (recovery of 50bp-30kb fragments also possible but yield will be lower)
  • DNA fragment recovery:
    • PCR clean-up: 90-99%
    • Gel extraction: 95%
  • Maximum binding capacity per spin column is 25mg
  • Extremely fast PCR clean-up processing time: 5-10 mins
  • Gel extraction processing time: <20 mins

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